Chronic myelogenous leukemia other diagnostic studies

Other diagnostic studies for chronic myelogenous leukemia include bone marrow aspiration and biopsy, lumbar puncture, and lymph node biopsy. Genomic PCR, southern blot assay, reverse transcriptase (RT) PCR, northern blot analysis, western blot analysis or immunoprecipitation can be helpful in the diagnosis of chronic myelogenous leukemia; the gold standard diagnostic test in chronic myelogenous leukemia is cytogenetic analysis.

Other diagnostic studies for chronic myelogenous leukemia include:^[1][2][3]

• Bone marrow aspiration and biopsy

• Used to detect and/or determine the type of leukemic cells

• Lumbar puncture

• Used to detect any metastasis to the cerebral spinal fluid (CSF)

• Lymph node biopsy
  • Used to detect and/or determine the type of leukemic cells
• Genomic PCR
  • Used to determine the exact breakpoints of the fusion genes
• Southern blot assay
  • Used to determine the exact breakpoints of the fusion genes
• Reverse transcriptase (RT) PCR
  • Used to detect BCR-ABL transcripts at the RNA level
• Northern blot analysis
  • Used to detect BCR-ABL transcripts at the RNA level
• Western blot analysis
  • Used to demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
• Immunoprecipitation
  • Used to demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl

• 
  Bone marrow aspiration and biopsy. After a small area of skin is numbed, a Jamshidi needle (a long, hollow needle) is inserted into the patient’s hip bone. Samples of blood, bone, and bone marrow are removed for examination under a microscope.^[4]

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