Mycobacterium intermedium

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Mycobacterium intermedium
Etymology:Latin, intermedium, meaning between, rapidly and slowly-growing mycobacteria.

Gram-positive, and nonmotile acid-fast coccobacilli (2.0µm x 2.6 µm).

Colony characteristics

• Eugonic, smooth and photochromogenic colonies (3–5mm in diameter) on Löwenstein-Jensen medium.

Physiology

• Growth on Löwenstein-Jensen medium at 22°C, 31°C, 37°C and 41°C, (optimal temperature between 31°C and 37°C), within 2-3 weeks.
• Susceptible to ethambutol and rifampin.
• Resistant to isoniazid and streptomycin.

Differential characteristics

• Phylogenetic position between rapidly and slowly-growing mycobacteria.
• M. asiaticum is phenotypically very similar, but can be distinguished by its growth at 22°C, and by its dysgonic growth.

• Pulmonary disease
• Biosafety level 2

• Repeatedly isolated from sputum from a patient with pulmonary disease.

Strain 1669/91 = ATCC 51848 = CCUG 37583 = CIP 104542 = DSM 44049 = JCM 13572

• Meier et al. 1993. Mycobacterium intermedium sp. nov. Int. J. Syst. Bacteriol., 1993, 43, 204-209.

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