Glucuronidases are enzymes that separate glucoronic acid molecules from other molecules by cutting glycosidic bonds. They are thus classified as glycoside hydrolases that cleave glucuronides.

In eukaryotes, glucuronidase is located in lysosomes and plays an important role in recycling cellular components by cleaving glucuronide moieties from proteins. Glucuronidase exhibits both endo-glycosidase and exo-glycosidase activities, meaning that it can cleave monosaccharides from the middle of a chain or from the end.

Preparations of beta-glucuronidase from the snail Helix pomatia are commercially available and referred to by the trademark Glusulase. These enzymes preparations are used to digest the ascus of the yeast Saccharomyces cerevisiae after sporulation.

E. coli is among the few bacteria that can synthezise glucuronidase, and this trait is commonly used to identify the bacterium.