Diffuse large B cell lymphoma laboratory tests

Diagnostic Work up for Diffuse Large B cell Lymphoma include Laboratory tests including Complete Blood count (CBC), Comprehensive Metabolic Panel, LDH and Uric acid Levels, Genetic testing, molecular diagnostic assays and immunophenotyping. Screening tests for Hepatitis B, Hepatitis C and HIV are usually recommended after diagnosis.

DLBCL require a complete work-up includes immunophenotypic analysis, cytogenetic analysis (conventional methods and FISH), and molecular diagnostic assays.

Laboratory tests of diffuse large B cell lymphoma include:^[1]

• Complete blood count (CBC)
• Comprehensive metabolic panel
• Blood chemistry studies including LDH and Uric acid
• Immunohistochemistry(IHC)
  • CD20, CD3, CD5, CD45, CD10, BCL-2, BCl-6, MYC, IRF-4/ MUM-1, Ki-67
• Flow Cytometry Panel (Establishing Reliable Diagnosis require IHC with or without Flow cytometry)
  • Kappa/ Lambda, CD3, CD5, CD45, CD10, CD19, CD20
• IHC for DLBCL subtypes:
  • Kappa/ Lambda, CD30, CD138, Cyclin/D1, ALK, HHV-8, SOX-11, EBER-ISH
• Cytology (for primary CNS lymphoma only)
• Genetic testing
  • 8q24/MYC translocations
  • Immunoglobulin genes clonally rearranged and hypermutated
  • Mutation of BCL6, MYC, PAX5, PIM1, RhoH/TTFn, TP53 genes
  • Translocations involving c-MYC, BCL6, and IgH genes

• Screening tests for HCV and HBV ( to assess risk of Reactivation following immunotherapy/ chemotherapy) are recommended.
• Screening test for HIV

Template:WikiDoc Sources