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Quenching (fluorescence)
Introduction
Overview
Overview
- For other uses, see Quenching (disambiguation).
Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen and the iodide ion are common chemical quenchers. Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.
Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain rubidium complexes allows the measurement of oxygen saturation in solution. Quenching and dequenching upon interaction with a specific molecular biological target is the basis for activatable optical contrast agents for molecular imaging.[1]
See also
See also
- Dark quencher, for use in molecular biology.
- Fluorescence resonance energy transfer, a phenomenon on which some quenching techniques rely
References
References
- ↑ Weissleder R, Tung CH, Mahmood U, Bogdanov A (1999). “In vivo imaging of tumors with protease-activated near-infrared fluorescent probes”. Nat. Biotechnol. 17 (4): 375–8. doi:10.1038/7933. PMID 10207887.