Selectable marker

An alternative to a selectable marker is a screenable marker, which allows the researcher to distinguish between wanted and unwanted cells.

Examples of selectable markers include:

• the Abicr gene ^[1]
• Neo gene from Tn5, which confers antibiotic resistance to geneticin^[2]

A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection. They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell. Selectable markers are often antibiotic resistance genes; bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material.

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