Sulfatases EC 3.1.6. are esterase enzymes which remove sulfate from a variety of substrates by hydrolyzing various sulphate esters.

The following sulphatases were shown to be structurally related based on their sequence homology^[1][2],^[3],

• cerebroside-sulfatase
• steryl-sulfatase
• arylsulphatase A EC 3.1.6.8 (ASA), a lysosomal enzyme which hydrolyzes cerebroside sulphate;
• arylsulphatase B EC 3.1.6.12 (ASB) which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate residues of dermatan sulphate;
• arylsulphatase C (ASD) and E (ASE); steryl-sulphatase EC 3.1.6.2 (STS), a membrane bound enzyme which hydrolyzes 3-beta-hydroxy steroid sulphates;
• iduronate 2-sulphatase EC 3.1.6.13 (IDS), a lysosomal enzyme that hydrolyzes the 2-sulphate groups from iduronic acids in dermatan sulphate and heparan sulphate;
• N-acetylgalactosamine-6-sulphatase EC 3.1.6.4, which hydrolyzes the 6-sulphate groups of the N-acetyl-D-galactosamine of chondroitin sulphate and D-galactose 6-sulphate units of keratan sulphate;
• N-sulphoglucosamine sulphohydrolase EC 3.10.1.1, the lysosomal enzyme that hydrolyses N-sulpho-D-glucosamine into glucosamine and sulphate;
• glucosamine-6-sulphatase EC 3.1.6.14 (G6S), which hydrolyzes the N-acetyl-D-glucosamine 6-sulphate units of heparan sulphate and keratan sulphate;
• N-sulphoglucosamine sulphohydrolase EC 3.10.1.1, the lysosomal enzyme that hydrolyses N-sulpho-D-glucosamine into glucosamine and sulphate;
• sea urchin embryo arylsulphatase EC 3.1.6.1;
• green algae arylsulphatase EC 3.1.6.1, which plays a role in the mineralization of sulphates; and
• arylsulphatase EC 3.1.6.1 from Escherichia coli, Klebsiella aerogenes and Pseudomonas aeruginosa.