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Sulforhodamine B

Introduction

Sulforhodamine B or Kiton Red (C27H30N2O7S2; Molecular Weight 558.66 grams/mole) is a fluorescent dye with uses spanning from L.I.F.(Laser Induced Fluorescense) to the quantification of cellular proteins of cultured cells. The red, solid, water soluble dye is primarily used as a polar tracer.

The dye absorbs 565nm light and emits 586nm light. It does not exhibit pH dependent absorption or fluorescence over the range of 3 to 10[1].

References

  1. ↑ Coppeta, Rogers (1998). “Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements”. Experiments in Fluids: 1–15, .

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