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Tetradecanoylphorbol-13-acetate response element gene transcriptions
Introduction
Associate Editor(s)-in-Chief: Henry A. Hoff
Activator protein 1 (AP-1) was first discovered as a 12-O-Tetradecanoylphorbol-13-acetate (TPA)-activated transcription factor that bound to a cis-regulatory element of the human metallothionein IIa (hMTIIa) promoter and SV40.[1]
Consensus sequences
Consensus sequences
The AP-1 binding site was identified as the TPA response element (TRE) with the consensus sequence 5’-TGA G/C TCA-3’.[2]
12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element (TRE) (TGAGTCA).[3]
Samplings
Samplings
Copying the consensus sequence for the DNA-binding factor (TRE): 3′-TGAGTCA-5′ or 3′-TGACTCA-5′ and putting the sequence in “⌘F” does not find either sequence in either A1BG promoter using nucleotide positions as can be found by the computer programs.
For the Basic programs testing consensus sequence 3′-TGA(G/C)TCA-5′ (starting with SuccessablesTRE.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesTRE–.bas, looking for 3′-TGA(G/C)TCA-5′, 0.
- negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesTRE-+.bas, looking for 3′-TGA(G/C)TCA-5′, 0.
- positive strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesTRE+-.bas, looking for 3′-TGA(G/C)TCA-5′, 0.
- positive strand in the positive direction (from ZNF497 to A1BG) is SuccessablesTRE++.bas, looking for 3′-TGA(G/C)TCA-5′, 0.
- complement, negative strand, negative direction is SuccessablesTREc–.bas, looking for 3′-ACT(C/G)AGT-5′, 0.
- complement, negative strand, positive direction is SuccessablesTREc-+.bas, looking for 3′-ACT(C/G)AGT-5′, 0.
- complement, positive strand, negative direction is SuccessablesTREc+-.bas, looking for 3′-ACT(C/G)AGT-5′, 0.
- complement, positive strand, positive direction is SuccessablesTREc++.bas, looking for 3′-ACT(C/G)AGT-5′, 0.
- inverse complement, 3′-TGA(C/G)TCA-5′ is the same as 3′-TGA(G/C)TCA-5′.
- inverse, 3′-ACT(C/G)AGT-5′ is the same as 3′-ACT(C/G)AGT-5′, the complement.
Acknowledgements
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
References
References
- ↑ Lee W, Haslinger A, Karin M, Tjian R (January 1987). “Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40”. Nature. 325 (6102): 368–72. Bibcode:1987Natur.325..368L. doi:10.1038/325368a0. PMID 3027570.
- ↑ Angel P, Imagawa M, Chiu R, Stein B, Imbra RJ, Rahmsdorf HJ, Jonat C, Herrlich P, Karin M (June 1987). “Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor”. Cell. 49 (6): 729–39. doi:10.1016/0092-8674(87)90611-8. PMID 3034432.
- ↑ Hiroshi Sato, Megumi Kita, and Motoharu Seiki (5 November 1993). “v-Src Activates the Expression of 92-kDa Type IV Collagenase Gene through the AP-1 Site and the GT Box Homologous to Retinoblastoma Control Elements” (PDF). The Journal of Biological Chemistry. 268 (31): 23460–8. Retrieved 13 August 2020.