List of molecular biology topics

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

This is a list of topics in molecular biology. See also list of biochemistry topics.

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3′ end – 3′ flanking region – 5′ end – 5′ flanking region – 5′-ribose- 3′ –

acrylamide gels – adenine – adenosine deaminase deficiency – adenovirus – agarose gel electrophoresis – agarose gel – Alagille syndrome – alkaline lysis – allele – amino acids – amino terminus – amp resistance – amplification – amplicon – anchor sequence – animal model – anneal – anti-sense strand – antibiotic resistance – antibody – antisense – antisense strand – AP-1 site – apoptosis – assembled epitope – ataxia-telangiectasia – ATG or AUG – autoimmune lymphoproliferative syndrome – autoradiography – autosomal dominant – autosome – avidin –

BAC – back mutation – bacteria – bacterial artificial chromosome – bacteriophage – bacteriophage lambda – band shift assay – base – base pair – binding site – biotin – birth defect – blotting – blunt end – bone marrow transplantation – box – BP – BRCA1 – BRCA2 –

C terminus – cancer – candidate gene – Canonical sequence – cap – cap site – carboxyl terminus – carcinoma – carrier – CAT assay – CCAAT box – cDNA – cDNA clone – cDNA library – cell – centimorgan – centromere – chain terminator – chaperone protein – chromosome – chromosome walking – CIS – cistron – clone (genetics) – clone (noun) – clone (verb) – cloning – coding sequence – coding strand – codon – codon usage bias – competent – complementary – conformational epitope – congenital – consensus sequence – conservative substitution – conserved – contig – cosmid – craniosynostosis – cystic fibrosis – cytogenetic map – cytosine – CpG

database search – degeneracy – deletion – denaturation – denaturing gel – deoxyribonuclease (DNase) – deoxyribonucleic acid – deoxyribonucleotide – diabetes mellitus – dideoxy sequencing – dideoxyribonucleotide – diploid – direct repeat – DNA ligase –DNA Bank – DNA polymerase – DNA replication – DNA sequencing – DNase – dominant – dot blot – double helix – downstream (molecular biology) – ds – duplex –

E. coli – electrophoresis – electroporation – Ellis-van Creveld syndrome – end labeling – endonuclease – enhancer – enzyme – epitope – ERE – ethidium bromide – evolutionary clock – evolutionary footprinting – exon – exonuclease – expression – expression clone – expression vector –

familial Mediterranean fever – fibroblasts – fluorescence in situ hybridization – footprinting – Fragile X syndrome – frameshift mutation – fusion protein –

gel electrophoresis – gel shift – gel shift assay – gene – gene amplification – gene conversion – gene expression – gene mapping – gene pool – gene therapy – gene transfer – genetic code – genetic counseling – genetic map – genetic marker – genetic screening – genome – genomic blot – genomic clone – genomic library – genotype – germ line – glycoprotein – glycosylation – Golgi apparatus – GRE – guanine –

hairpin – haploid – haploinsufficiency – helix-loop-helix – hematopoietic stem cell – hemophilia – heteroduplex DNA – heterozygous – highly conserved sequence – Hirschsprung’s disease – histone – hnRNA – holoprosencephaly – homologous recombination – homology – homozygous – host strain (bacterial) – human artificial chromosome – Human Genome Project – human immunodeficiency virus – Huntington’s disease – hybridization – hybridoma – hydrophilicity plot –

immunoblot – immunoprecipitation – immunotherapy – in situ hybridization – in vitro translation – inducer – inherited – initiation codon – insert – insertion – insertion sequence – intellectual property rights – intergenic – intron – inverted repeat –

Junk DNA

karyotype – kilobase – kinase – Klenow fragment – Knock-down – knock-out – knock-out experiment – knockout – Kozak sequence

lambda – leucine zipper – leukemia – library – ligase – linear epitope – linkage – linker – lipofectin – locus – LOD score – lymphocyte –

M13 phage – malformation – mapping – marker – melanoma – melting – Johann Mendel – Mendelian inheritance – message – messenger RNA – metaphase – microarray technology – microsatellite – missense mutation – mitochondrial DNA – mobility shift – molecular weight size marker – monoclonal antibody – monosomy – mouse model – mRNA – multicistronic message – multicopy plasmid – multiple cloning site – multiple endocrine neoplasia, type 1 – mutation –

N terminus – native gel – nested PCR – neurofibromatosis – nick (DNA) – nick translation – Niemann-Pick disease, type C – non-coding DNA – non-coding strand – non-directiveness – nonconservative substitution – nonsense codon – nonsense mutation – nontranslated RNA – Northern blot – NT – nuclear run-on – nuclease – nuclease protection assay – nucleoside – nucleotide – nucleus –

oligo – oligodeoxyribonucleotide – oligonucleotide – oncogene – oncovirus – open reading frame – operator – operon – origin of replication – overhang –

p53 – package – palindromic sequence – Parkinson’s disease – pBR322 – PCR – pedigree – peptide – peptide bond – phage – phagemid – phenotype – phosphatase, alkaline – phosphodiester bond – phosphorylation – physical map – plasmid – point mutation – poly-A track – polyA tail – polyacrylamide gel – polyclonal antibodies – polydactyly – polymerase – polymerase chain reaction – polymorphism – polynucleotide kinase – polypeptide – positional cloning – post-transcriptional regulation – post-translational modification – post-translational processing – post-translational regulation – PRE – Precursor mRNA – primary immunodeficiency – primary transcript – primer – primer extension – probe – processing – processivity – promoter – pronucleus – prostate cancer – protease – protein – proto-oncogene – pseudogene – pseudoknot – pseudorevertant – pulse sequence database – pulsed field gel electrophoresis – purine – pyrimidine

random primed synthesis – reading frame – recessive – recognition sequence – recombinant DNA – recombination – recombination-repair – relaxed DNA – repetitive DNA – replica plating – reporter gene – repression – repressor – residue – response element – restriction – restriction endonuclease – restriction enzyme – restriction fragment – restriction fragment length polymorphism (RFLP) – restriction fragments – restriction map – restriction site – reticulocyte lysate – retrovirus – reverse transcriptase – reverse transcription – revertant – ribonuclease – ribonuclease – ribonucleic acid – riboprobe – ribosomal binding sequence – ribosome – ribozyme – risk communication – RNA polymerase – RNA splicing – RNAi – RNase – RNase protection assay – rRNA – RT-PCR – run-off – Run-on – runoff transcript

S1 end mapping – S1 nuclease – screening – SDS-PAGE – secondary structure – selection – sense strand – sequence – sequence motif – sequence polymorphism – sequence-tagged site – sequential epitope – severe combined immunodeficiency – sex chromosome – sex-linked – Shine-Dalgarno sequence – shotgun cloning – shotgun cloning or sequencing – shotgun sequencing – shuttle vector – sickle-cell disease – side chain – sigma factor – signal peptidase – signal sequence – silent mutation – single nucleotide polymorphism – siRNA – site-directed mutagenesis – site-specific recombination – slot blot – SNP – snRNA – snRNP – solution hybridization – somatic cells – Southern blot – southwestern blot – SP6 RNA polymerase – spectral karyotype – splicing – Simple Sequence Repeats (SSR) – stable transfection – start codon – stem-loop – sticky end – stop codon – streptavidin – stringency – structural motif – sub-cloning – substitution – suicide gene – supercoil – syndrome –

T7 RNA polymerase – taq polymerase – TATA box – technology transfer – template – termination codon – terminator – tertiary structure – tet resistance – thymine – tissue-specific expression – tm – trans – transcript – transcription – transcription factor – transcription/translation reaction – transcriptional start site – transfection – Transformation (genetics) – transformation (with respect to bacteria) – transfection (with respect to cultured cells) – transgene – transgenic – transgenic mouse – transient transfection – transition – translation – translocation – transposition – transposon – transversion – triplet – trisomy – tRNA – tumor suppressor – tumor suppressor gene –

untranslated RNA – upstream – upstream activator sequence – upstream (molecular biology) – uracil –

vector –

Western blot – wildtype – wobble position – Wolfram syndrome –

YAC (yeast artificial chromosome)

zinc finger –

• List of biochemistry topics

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