Splenic marginal zone lymphoma laboratory findings

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Muhammad Affan M.D.[2], Sowminya Arikapudi, M.B,B.S. [3]

Overview

Laboratory tests for splenic marginal zone lymphoma (SMZL) include complete blood count (CBC), blood chemistry studies, immunohistochemistry, genetic testing, flow cytometry, FISH, PCR, and immunophenotyping.

Laboratory tests

Laboratory tests for splenic marginal zone lymphoma (SMZL) include:^[1]

Complete blood count (CBC) with differential count
- Pancytopenia or leukemic presentation with leukocytosis, anemia, thrombocytopenia^[2]
Serum albumin levels
serum LDH levels
Beta-2-microglobulin
Immunohistochemistry^[3]
- Typically positive for CD20,CD79a, BCL-2 and immunoglobuin M (IgM).
- Surface immunoglobulin D (IgD) and DBA44 are variably expressed.
- Negative for CD5, CD10, CD43, CD103, CD123, Annexin A1, BCL-6 and LEF-1.
Genetic testing^[4]
- Chromosomal 7q32 deletion (30-40%).
- Chromosome 3q gains (20-30%)).
- NOTCH2 and KLF2 are the commonly reported gene mutations.
Flow cytometry^[5]
FISH (fluorescence in situ hybridization)^[6]
PCR^[7]

References

↑ Splenic marginal zone lymphoma. Surveillance, Epidemiology, and End Results Program 2015. http://seer.cancer.gov/seertools/hemelymph/51f6cf57e3e27c3994bd5327/ Accessed on December 22,2015
↑ Yang SM, Jiang Q, Jiang B, Chen DB, Wang J, Jiang H, Lu J, Lu XJ, Bao L, Shi HX, Liu YR, Huang XJ (February 2013). “[Clinical characteristics of splenic marginal zone lymphoma with abnormal complete blood count]”. Zhongguo Shi Yan Xue Ye Xue Za Zhi (in Chinese). 21 (1): 87–94. doi:10.7534/j.issn.1009-2137.2013.01.019. PMID 23484698.
↑ Piris MA, Mollejo M, Campo E, Menárguez J, Flores T, Isaacson PG (September 1998). “A marginal zone pattern may be found in different varieties of non-Hodgkin’s lymphoma: the morphology and immunohistology of splenic involvement by B-cell lymphomas simulating splenic marginal zone lymphoma”. Histopathology. 33 (3): 230–9. PMID 9777389.
↑ Parry M, Rose-Zerilli MJ, Ljungström V, Gibson J, Wang J, Walewska R, Parker H, Parker A, Davis Z, Gardiner A, McIver-Brown N, Kalpadakis C, Xochelli A, Anagnostopoulos A, Fazi C, de Castro DG, Dearden C, Pratt G, Rosenquist R, Ashton-Key M, Forconi F, Collins A, Ghia P, Matutes E, Pangalis G, Stamatopoulos K, Oscier D, Strefford JC (September 2015). “Genetics and Prognostication in Splenic Marginal Zone Lymphoma: Revelations from Deep Sequencing”. Clin. Cancer Res. 21 (18): 4174–4183. doi:10.1158/1078-0432.CCR-14-2759. PMC 4490180. PMID 25779943.
↑ Hu Y, Chen Y, Wang LH, Chen X, Fang F, Liu SQ, Wu XQ, Zhu P (April 2014). “[Identification of splenic marginal zone lymphoma from B lymphoproliferative disorders by flow cytometry]”. Zhongguo Shi Yan Xue Ye Xue Za Zhi (in Chinese). 22 (2): 349–56. doi:10.7534/j.issn.1009-2137.2014.02.016.
↑ Yi S, Yan Y, Xiong W, Lv R, Yu Z, Liu W, Liu E, Li H, Liu H, Li Z, An G, Xu Y, Ru K, Zou D, Qiu L (November 2017). “Distinct clinical characteristics draw a new prognostic model for splenic marginal zone lymphoma in HBV high prevalent region”. Oncotarget. 8 (58): 98757–98770. doi:10.18632/oncotarget.21931. PMC 5716765. PMID 29228725.
↑ Arribas AJ, Rinaldi A, Mensah AA, Kwee I, Cascione L, Robles EF, Martinez-Climent JA, Oscier D, Arcaini L, Baldini L, Marasca R, Thieblemont C, Briere J, Forconi F, Zamò A, Bonifacio M, Mollejo M, Facchetti F, Dirnhofer S, Ponzoni M, Bhagat G, Piris MA, Gaidano G, Zucca E, Rossi D, Bertoni F (March 2015). “DNA methylation profiling identifies two splenic marginal zone lymphoma subgroups with different clinical and genetic features”. Blood. 125 (12): 1922–31. doi:10.1182/blood-2014-08-596247. PMC 4416938. PMID 25612624.

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[”seer”-1] Splenic marginal zone lymphoma. Surveillance, Epidemiology, and End Results Program 2015. http://seer.cancer.gov/seertools/hemelymph/51f6cf57e3e27c3994bd5327/ Accessed on December 22,2015

[pmid23484698-2] Yang SM, Jiang Q, Jiang B, Chen DB, Wang J, Jiang H, Lu J, Lu XJ, Bao L, Shi HX, Liu YR, Huang XJ (February 2013). “[Clinical characteristics of splenic marginal zone lymphoma with abnormal complete blood count]”. Zhongguo Shi Yan Xue Ye Xue Za Zhi (in Chinese). 21 (1): 87–94. doi:10.7534/j.issn.1009-2137.2013.01.019. PMID 23484698.

[pmid9777389-3] Piris MA, Mollejo M, Campo E, Menárguez J, Flores T, Isaacson PG (September 1998). “A marginal zone pattern may be found in different varieties of non-Hodgkin’s lymphoma: the morphology and immunohistology of splenic involvement by B-cell lymphomas simulating splenic marginal zone lymphoma”. Histopathology. 33 (3): 230–9. PMID 9777389.

[pmid25779943-4] Parry M, Rose-Zerilli MJ, Ljungström V, Gibson J, Wang J, Walewska R, Parker H, Parker A, Davis Z, Gardiner A, McIver-Brown N, Kalpadakis C, Xochelli A, Anagnostopoulos A, Fazi C, de Castro DG, Dearden C, Pratt G, Rosenquist R, Ashton-Key M, Forconi F, Collins A, Ghia P, Matutes E, Pangalis G, Stamatopoulos K, Oscier D, Strefford JC (September 2015). “Genetics and Prognostication in Splenic Marginal Zone Lymphoma: Revelations from Deep Sequencing”. Clin. Cancer Res. 21 (18): 4174–4183. doi:10.1158/1078-0432.CCR-14-2759. PMC 4490180. PMID 25779943.

[pmid-5] Hu Y, Chen Y, Wang LH, Chen X, Fang F, Liu SQ, Wu XQ, Zhu P (April 2014). “[Identification of splenic marginal zone lymphoma from B lymphoproliferative disorders by flow cytometry]”. Zhongguo Shi Yan Xue Ye Xue Za Zhi (in Chinese). 22 (2): 349–56. doi:10.7534/j.issn.1009-2137.2014.02.016.

[pmid29228725-6] Yi S, Yan Y, Xiong W, Lv R, Yu Z, Liu W, Liu E, Li H, Liu H, Li Z, An G, Xu Y, Ru K, Zou D, Qiu L (November 2017). “Distinct clinical characteristics draw a new prognostic model for splenic marginal zone lymphoma in HBV high prevalent region”. Oncotarget. 8 (58): 98757–98770. doi:10.18632/oncotarget.21931. PMC 5716765. PMID 29228725.

[pmid25612624-7] Arribas AJ, Rinaldi A, Mensah AA, Kwee I, Cascione L, Robles EF, Martinez-Climent JA, Oscier D, Arcaini L, Baldini L, Marasca R, Thieblemont C, Briere J, Forconi F, Zamò A, Bonifacio M, Mollejo M, Facchetti F, Dirnhofer S, Ponzoni M, Bhagat G, Piris MA, Gaidano G, Zucca E, Rossi D, Bertoni F (March 2015). “DNA methylation profiling identifies two splenic marginal zone lymphoma subgroups with different clinical and genetic features”. Blood. 125 (12): 1922–31. doi:10.1182/blood-2014-08-596247. PMC 4416938. PMID 25612624.

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